For those few who know me you know I have been taking a Microbiology courseover Summer A.  Today I finished my final and got my final grade, 89.9%.  I will have to take time to regenerate my system from all the stress of these last 6 weeks but I am happy with a B+.

I will be back up and running soon but till then I will be Sleeping, playing Spore, and playing World of Warcraft.  Hope everyone else who has taken summer had as good a time as I did.

by Dr. Arian Azarbar

Following trying different media (potato slices, gelatin, etc) he used a cooking thickener (agar) which produced a company surface area so the bacteria could be spread very thinly over the surface.

The micro organism is spread so thinly that person organisms are separated from each other. Then after time, each person organism would multiply to form a visible colony from its millions of descendents.

Koch's discovery of the method of getting ready pure culture of bacteria permitted German microbiologists to advance nicely ahead of microbiologists of France and other nations who had been still using broth culture and successive dilutions to get a simple organism.

Other work attributed to Koch includes the discoveries of Vibrio cholera in 1883 and the tuberculosis bacterium in 1882 and his studies in tuberculosis later on resulted in his winning the Nobel Prize in Physiology or Medication in 1905.

He is also recognized for his function in Africa with Trypanosoma and tick borne spirochetes.

But what numerous believe his best contribution in microbiology and infectious diseases is his formulation of four postulates that affiliate a specific organism with a particular disease.

Koch's postulates are as following:

one. The particular causative agent must be discovered in each case of the illness.
2. The illness organism should be isolated in pure tradition.
three. Inoculation of a sample of the culture into a healthy, susceptible bestial must produce the exact same illness.
four. The disease organism should be recovered from the inoculated animal.

Implied in Koch's postulates are the one organism-1 disease concept and it supplied the method of establishing the germ concept of illness.

Robert Koch had numerous other accomplishments in the field of microbiology and infectious illnesses and was well respected on the continents of Africa and Asia for his function.

He died on Might 27, 1910 from a heart assault.

Screening laboratories in the type of a microbiology lab is the office where health-related advancements testings are held. With this, they can arrive up with health-related discoveries this kind of as new medication and medicines for various illnesses.

Because these screening laboratories are very important for discovering new health-related developments, it is just correct for people who are in the laboratory to know the greatest way of maintaining their microbiology lab. This will help them properly do the check and arrive up with the best result that will be a good assist for them in their research. The subsequent are the methods that they ought to do in the lab.

First, it is important for them to know the greatest way of preparing the check equipments. This will make sure them that they will maintain their check tradition from becoming contaminated right at the starting of the test. Most of the time, contamination also occur at the initial components of the check simply because of incorrect planning.

Second, they should deal with the test culture correctly during the flow of screening. With this, they will keep their tradition safe from contaminants that will wreck the outcome of the check. They ought to know exactly where they ought to maintain the test tradition from dirt and other variables that will alter the test results.

Proper microbiology lab upkeep is the 3rd thing to do. Microbiology

  Today, I had a microbiology class at VCU's life sciences center, and it was really fun.  Not only that, but while it's a class, you have no homework or tests!    The bus ride home was very bumpy, and so went I got out at the bus stop, I staggered a bit getting off because I was so disoriented!  XD  Afterwards, when Dad picked me up, we went to a New York-style deli where we had this huge cornbeef sandwhich with potato salad (that wasn't very good, unfortunately) and a bowl of sour kosher dill pickles.  Unfortunately, they didn't have kinishes.  It was enjoyable though, and my stomach felt all warm and fuzzy afterwards.  ^^  Also, I've noticed I've gotten more and more lazy into the school year and my grades in English are dropping (not because I don't study, but on the essay reading quizzes he gives us, I either don't understand the story or completely forget the main character's name!).  I also have a report to write on a speech that Mark Twain gave, so I've got to get started on that (not to mention to continue to seriously edit my contest fanfic).  I've just been so worried this year.  

I'll update this as the month goes on and more microbiology stuff goes on.

  Two days ago was my second microbiology class!  They couldn't do it last week because of a "weather emergency" (even though it didn't snow that day; just cloudy).  Anyways, I did better on the bus this time and we had corn beef sandwiches again, and this time, I actually all of my half (I split it with Dad because there was no way I was going to be able to eat all of it)!    I was so stuffed afterwards that I didn't eat much dinner.    Can't wait until next week when I get to eat another one (it's become a post-microbiology ritual right now)!  Yup, Dad's finally done it!  He's turned me into a New York-style cornbeef sandwich freak!  

Well, toodles!



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You can perform it, but you can't explain it at times.
That's the feeling I had when I did my tests..
Besides that, I will upload some pictures I took today later tonight as soon as I collect my syringe pens. Yes.. pens that are syringe shaped.. ^_^

Well had the exam monday and i was only confident i answered two questions correctly...Well one while sitting ther two after wards when people started talking...

The results were posted rather quickly and i had an A- well that really really shocked me as i completely blanked on the fact that pasteur was responsible for pasterisation 'sigh'

Just waiting for Essay results now seeing as i got an A for the work book which i'm really happy with

This is awesome! I was reading in my microbiology textbook about the four phases of celluar batch growth: lag, exponential, stationary, and death. It seemed common sensical that cells from an exponential phase being transplanted into an equally good fresh media will skip the lag phase and continue growing exponentially. What floored me was that cells from an exponential phase transplanted into a poor media (or cells from a stationary phase transplanted into an equally good fresh media) will go into lag before going into exponential! Isn't that weird!?! Why don't cells jump right into exponential growth immediately upon finding themselves in a nutritional paradise?

i.e. To me, this was like imagining the pilgrims on the Mayflower. I would have thought (incorrectly) that after being seasick for several months straight, when they landed on the New World with limitless timber and fresh water that the pilgrims would have sprung to life overnight. Instead, upon hitting Plymouth Rock, imagine them just griping and floundering about for a few years (lag phase) prior to populating the entire continent (exponential) with their colonies.  

Apparently, the book's explanation is that those cells require time to repair themselves instead of just leaping into high gear instantly. (I guess it makes sense that sex isn't on a cell's mind when it's starving or on teeny 'lil crutches.) Yet, it's amazing that even in good times a microorganism doesn't thrive right away. This is a major epiphany to me! LOL

Yet, it makes sense when I think about it on a macro-scale like with pilgrims from the Mayflower. Another example that came to mind is from Sun Tzu's Art of War, where the B.C. Chinese general says to the reader that any culture created on conquest requires conquest to sustain itself. i.e. Once you start conquering other lands, you're in exponential phase and find it easy to continue conquering because you're like exponential cells from a good media transplanted into an equally good media, but when you can no longer sustain your expansionist foreign policy of conquest, your let-the-good-times-roll culture goes into the stationary phase. Likewise, a culture from a nutrient-poor media like say, mainland Europe immediatley after WWII, requires a lag phase even though the Nazis were no longer a danger to anyone after 1945! Coooool! I think I'm onto something big here!

I have a practical assesment due today, oh woe is me, it's actually quite difficult mainly because it was rather awkward to listen during the practicals and take notes when you're trying desperately not to spill E.Coli (i failed btw, it was an agary mess )

So now i've got a set of questions i'm not quite sure i can answer for a sheet worth 20% of my grade......

Darn.



Wish me luck guys (luck for getting the answers off someone else that is .. just kidding )

To those who are/were waiting on Critiques and/or a sketch from me, I have to apologize. I won't be able to do them. I've been having such an impossibly hard time with my schedule and my immense work load as of late that I can barely find time to relax anymore.

Honestly there are some days--like today--when I wonder why I worked so hard to get into CLS. I can understand why Gabrielle quit the program. My micro professor is absolutely horrible.

Me: I hate micro. I honestly, truly, deeply wish I could get my degree without it. The sheer, overwhelming amount of information she expects us to understand is just too much. Even the Seniors don't use half of what she's making us learn. Maybe I'd like it more if we had a different professor. Even the other prof's want her to cut down on our work--but NO, she refuses. FML.
20 September at 18:03

Gabrielle: That woman is the reason I'm not in the program anymore. She's foul.
        20 September at 18:18

Me: I know. She's so rude, too. You can't even ask a question without her making you feel like a moron. Today I wanted to compare my Na hippurate results with what I should have gotten and she got on my case about it. It's horrible. When I know I have micro that day I just get so stressed out. I haven't been sleeping well and I cry almost every day because of the expectations she's heaping on us. "Just know everything!" That isn't even POSSIBLE.
        20 September at 19:38

Ashton:  My roommate had her last year and hated her too. She is so glad to be a senior
        20 September at 19:42

Me: I bet. I can't wait to be a Senior. At least my favorite professor is also my advisor. Small miracles.
        20 September at 19:44


I was sick all weekend and didn't do much studying. I went over the objectives and pertinent charts yesterday and this morning, thinking I'd be okay.... well, I took one look at the test and wanted to just give up right then. I tried my best through the multiple guess and matching but when I got to the essays... I just started to cry (quietly). I didn't know how to answer any of them.

Not a single one.

So I just turned in what I had. I'll probably get a 10 or something and need a conference with the professors once it's graded. I just.. I always feel so defeated. I literally just want to sit down and cry every night because of how impossibly hard that class is. I'm not happy. I think more and more about how I want to just quit the program...

I feel more and more like it's hopeless. No class has ever made me hate myself as much or as deeply as this class. A class shouldn't make it's students feel clinically depressed, right?


<sub>THIS IS MY SCHEDULE. IF I AM SLOW ABOUT UPDATING/REPLYING, THIS IS WHY.

Monday
EXAMS 8:30 am - 11:30 am
Hematology I Laboratory CLSC 3411-001 1:15 pm - 4:30 pm

Tuesday
Hematology I  CLSC 3410-001 9:00 am - 9:50 am
Clinical Microbiology I  CLSC 4460-001 10:00 am - 11:50 am
Clinical Microbiology I Lab CLSC 4461-001 1:15 pm - 4:30 pm

Wednesday
Hematology I  CLSC 3410-001 9:00 am - 9:50 am
Clinical Immunology  CLSC 3430-001 10:00 am - 11:50 am
Clinical Chemistry Lab I  CLSC 4431-001 1:15 pm - 4:30 pm

Thursday
Clinical Chemistry I  CLSC 4430-001 9:00 am - 10:50 am
Clinical Microbiology I Lab CLSC 4461-001 1:15 pm - 4:30 pm

Friday
Hematology I  CLSC 3410-001 9:00 am - 9:50 am
Clinical Microbiology I  CLSC 4460-001 10:00 am - 11:50 am
Clinical Microbiology I Lab CLSC 4461-001 1:15 pm - 4:30 pm</sub>



Begin Turnabout.

In 1885, a nine year old boy who was seriously mauled by a rabid dog and his mom showed up to Pasteur's lab. The boy, who was doomed to die the horrible demise from rabies, was the initial individual to get immunized against rabies. He survived and remained in great well being for the rest of his existence. Pasteur became a legend as newspapers from about the globe noted this and other people saved by his rabies vaccine.

The list could go on and on with Louis Pasteur. He later on grew to become director of the famous Pasteur Institute in 1894. He died the next yr as a nationwide hero.

Light microscopy is the most largely used microscopy method in microbiology labs. In mild (optic) microscopy, the objects studied appear darkened and the adjacent region appears very nicely illuminated. The magnification energy of an optic microscope is up to 2000 times. The primary trouble in mild microscopy is to acquire a substantial magnification energy along with clearness and picture definition.

In light microscopy, the resolution power represents the dimension of the smaller sized object that can be noticed on the microscope with complete clearness. A great microscope shall mix magnification power with resolution energy or else an picture could be amplified 2000 occasions, but it would show up distorted and without definition.

The lower the resolution energy, smaller the dimension of the object that can be observed on the microscope. That stated, a light microscope with a resolution energy of .five &microm is in a position to magnify, with clearness, objects of .five &microm or greater.

In order to calculate the resolution power of an optic microscope, we need to know:

• The light wavelength


• The objective lenses numeric aperture


• The condenser lens numeric aperture.

The wavelength of the visible mild is recognized and ranges from 400 to 700 nm, or .4 to .7 &microm.

In mild microscopy, the Numeric Aperture (NA) is calculated from the subsequent equation:

NA = n sen&theta, where

n = the medium refraction index. n=1.56 when an immersion oil is used and n=1 when it is not utilized.

&theta = fifty percent angle of the cone light that enters the objective or condenser lenses.

In the modern microscopes, the NA value of the goal and condenser lenses is indicated at the microscope user's guide.

At final, the resolution energy (RP) is calculated from the subsequent equation:

RP = light wavelength/(NA goal + NA condenser)

At microbiology labs, the mild microscopy method is generally performed utilizing immersion oil. The immersion oil has a refraction index higher than the air, what raises NA and decreases RP in sensible terms, it allows the observation of smaller sized objects, like cells and bacteria, for example.

The polymerase chain reaction (PCR) is a procedure for amplifying very tiny amounts of DNA such that they can be visualized and assessed or utilized in additional scientific processes. PCR is widely utilized in nearly all branches of biology including molecular biology, microbiology, genetics, environmental science, meals science, biotechnology, forensic science, and clinical diagnostics. The PCR method entails using an enzyme called DNA polymerase to amplify (duplicate many occasions) a piece of DNA. The original molecule of DNA is duplicated by the DNA polymerase enzyme, thus doubling the quantity of DNA molecules.

Arian Azarbar